Isolation, identification and soil remediation of atrazine-degrading strain T3AB1

Abstract:

Abstract：[Objective] To provide new atrazine-degrading strains for atrazine-polluted soil, we isolated the high-efficiency degradation bacterium from contaminated soil, identified with taxonomy, and studied the degrading characteristics and remediation capability of the strain in black soil. [Methods] We used morphological, physiological and biochemical characteristics and 16S rDNA sequences analysis to identify the strain. We studied the effect of environmental factors such as incubation time, temperature and pH on the strain growth and atrazine degradation to confirm the optimum degradation conditions. The strain’s degradation range was studied by using different herbicides as sole carbon and nitrogen source. The strain’s remediation capability to atrazine-polluted soil was tested by sensitive crop bioassay after inoculating in pot soil. [Results] We isolated a high-efficiency degradation strain T3AB1 that used atrazine as sole carbon and nitrogen source from maize field suffering atrazine in Nehe, Heilongjiang province. It was initially identified as a member of the genus Arthrobacter sp.. The strain could degrade more than 99% of 500mg/L atrazine (pH8.0) within 72 h. We determined the best degradation conditions as follows: pH7.0－8.0, 25－30℃, 72－108h. In addition, the strain used other herbicides as sole carbon and nitrogen source such as imazamox, imazethapyr, trifluralinm, clomazone and fomesafen, and the degradation rate reached 12.66%－40.54% after 168h. The strain could significantly remedy the sensitive rice crop biomass indexes after treating 21 days, and as time extending, its role in the remediation gradually increased. [Conclusion] The screened atrazine-degrading strain T3AB1 could be a suitable candidate for bioremediation of atrazine-polluted soil.

Key words:Keywords：atrazine; biodegradation; Arthrobacter sp.; soil remediation