Effect of transglutaminase-gene disruption in Streptomyces hygroscopicus on cell differentiation

Abstract:

Abstract: [Objective]To provide theoretical basis for understanding the physiological function of transglutaminase in Streptomyces, which would benefit for improving the production of this enzyme, transglutaminase gene was disrupted in Streptomyces hygroscopicus.【Methods】First, the interruption plasmid pKC1139-TG1 was constructed by inserting a segment of transglutaminase gene in the temperature-sensitive vector pKC1139. Second, pKC1139-TG1 was transformed into S. hygroscopicus protoplast and the interruption plasmid was inserted into the chromosome by homogenous recombination .Third, the strain with disrupted transglutaminase gene was screened by apramycin resistance and named as S. h-△TG. 【Results】Compared with the parent strain, S. h-△TG was able to grow as substrate mycelium, but not able to form aerial hyphae.【Conclusion】Transglutaminase may be involved in the aerial hyphae formation of S. hygroscopicus.

Key words:Keywords: Streptomyces hygroscopicus; transglutaminase; Gene disruption; Mycelium differentiation