Influence of liquid or solid culture conditions on the volatile components of mycelia of Isariacateinannulata

Abstract:

Abstract:［Objective］To determine the volatile components of mycelia of Isaria cateinannulata cultured under different culture conditions，and to analyze the relationships between the culture conditions and volatile metabolites．［Methods］Mycelia were cultured in solid plates with SDAY medium and liquid shake flasks with SDY medium．The culture conditions were at 25℃ and 8 days． Volatile components in the mycelia of I． cateinannulata were extracted with simultaneous distillation extraction and analyzed by gas chromatography-mass spectrometry． ［Result］Alkenes，alkanes，heterocyclic and polycyclic aromatic hydrocarbons( PAH) were existed abundantly both in the mycelia of liquid and solid cultures，but the kinds and relative concentrations of the volatile components in mycelia of liquid and solid cultures were very different． Forty-one compounds were identified from the mycelia of solid culture and 32 compounds were identified from the mycelia of liquid culture． Esters，quinones and oximes were only found in solid cultured mycelia whereas carboxylic acids were only discovered in the mycelia of liquid culture． At the same time，mycelia of liquid culture contained much more phenols． The most abundant compounds in mycelia of liquid and solid cultures were hydrocarbons．The volatile extracts of solid cultured mycelia contained 57. 6% alkenes and 9. 19% alkanes． The volatile extracts of liquid cultured mycelia contained 7. 85% alkenes and 22. 4% alkanes． ［Conclusion］ Liquid or solid culture conditions influenced the volatile components of mycelia of I． cateinannulata．

Key words:Keywords: Isaria cateinannulata，liquid culture，solid culture，mycelia，gas chromatography-mass spectrometry