A new method of microbial prospecting for oil and gas based on BIOLOG metabolic fingerprinting analysis

Abstract:

Abstract:［Objective］Establishing a new method of microbial prospecting for oil and gas based on microbial community metabolic function． ［Methods］In this study，45 shallow reservoir soil samples and 25 non-reservoir soil samples were collected． Carbon catabolic activity of the microbial community was measured by biolog microplate，and microbial anomaly of reservoir was judged by discriminant function． ［Result］We screened 10 typical carbon sources of 95 carbon sources which could reflect the differences of reservoir and non-reservoir soil microbial community structure and made the validation test set to experimental zone and controlled zone samples using the discriminant function． The discriminate rate of reservoir and non-reservoir were 97. 8% and 100% respectively and the overall accuracy was 98.6%.［Conclusion］ Biolog technology provided a highly efficient and precise method for preliminarily predicting oil and gas reservoir．