Quantity of Desulfovibrios and analysis of intestinal microbiota diversity in health and intestinal disease people in Wuxi，Jiangsu province

Abstract:

Abstract:［Objective］This paper provides an overview of Desulfovibrio (DSV) incidence and its effect on bacterial diversity in human gastrointestinal tract of four groups: ulcerative colitis (UC)，colorectal cancer (CRC)，polypus (PP) and the healthy control (H)．［Methods］ Real time fluorescence quantitative PCR (RT-PCR) assays were used to enumerate DSV in gastrointestinal tract of 58 subjects． Diversity of gut microbiota was analyzed by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) and 16S rRNA V3 sequencing．［Results］ RT-PCR detected DSV in all samples． Significantly increased numbers of DSV were observed for UC and PP groups compared with CRC and H groups．No significant difference was observed for CRC and H groups with gene copy numbers of DSV． Alterations of DSV and gut microbiota were observed in disease groups． ［Conclusion］ We found that quantity and diversity of DSV are significantly increased in UC and PP compared to controls． The increased numbers of DSV in disease groups suggests a possible harmful role．

Key words:Keywords: real time PCR，DGGE，Desulfovibrio，intestinal diseases，16S rRNA，dsrB