Denaturing gradient gel electrophoresis analysis on bacterial community change in the phase Ⅱ composting of Volvariella volvacea substrate

Abstract:

Abstract:［Objective］To study the bacterial community change during the phase II composting of Volvariella volvacea substrate of cotton waste and to clarify the predominant bacteria at different composting stages． Methods］ The 16S rDNA-DGGE (denaturing gradient gel electrophoresis) and clone strains sequencing methods were used to track the change of bacterial community during composting．［Results］ The DGGE profile shows that the diversity of bacterial community was rich and bands diversity decreased with the composting progress． The dynamic changes of predominant community and relative intensity were observed． The 23 predominant strains belong to 3 classes of Proteobacteria，Bacteroidetes and Firmicutes，6 families of α，β，γ-Proteobacterium，Bacteroidetes，Sphingobacteria and Clostridia，11 genera，in which 19 strains were thermophilic bacteria． The genera of Stenotrophomonas，Comamonas，Sphingobacterium and Sinorhizobium were predominant bacteria in the stages of high temperature and drop of temperature during composting．［Conclusion］The bacterial community structure and predominant community change dynamically during the phase II of Volvariella volvacea composting，especially in the course of entering the stage of high temperature．

Key words:Keywords: Volvariella volvacea，phase II of composting，bacterial community，16S rDNA-DGGE