Syntrophic effects of two species of maine obligate hydrocarbonoclastic bacteria in oil biodegradation process

Abstract:

Abstracts: [Objective] In order to study the mutual effects of marine obligate hydrocarbonoclastic bacteria in the oil biodegradation process, [Methods] we utilized Alcanivorax sp. 22CO-6, JZ9B and Marinobacter sp. PY97S to construct oil-degrading consortia. Multiple methods including weighting method, GC-FID, GC-MS and TLC-FID were used to analyze and compare the oil degradation rates as well as the chromatographic figures of degraded oil between OHCB pure cultures and defined consortia. [Results] The two OHCB species in the defined consortia 22CO-6+PY97S and JZ9B+PY97S exhibited strong syntrophic effects in the oil biodegradation process. And the degradation rates of oil were increased from 27.81% and 83.52% to 64.03% and 86.89%, respectively. The consortia could degrade aliphatic and aromatic fraction at the same time, including high molecular weight-PAHs chrysene and its alkyl derivatives. [Conclusion] There are obvious syntrophic effets between marine OHCBs Alcanivorax and Marinobacter strains in the oil biodegradation process, which could not only accelerate the oil biodegradation, but also decompose thoroughly the more ecotoxic high molecular weight compounds in crude oil.

Key words:Keywords: obligate hydrocarbonoclastic bacteria; syntrophic effects; oil spill; biodegradation; Polycyclic aromatic hydrocarbons