Feng Gao
College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, ChinaBin Wang
College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, ChinaTaichun Chen
College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, ChinaZhijun Wang
Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiaotong University, Shanghai 200030, ChinaDerong An
College of Plant Protection and Shanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A&F University, Yangling 712100, ChinaSupported by the National Programs for High Technology Research and Development of China (2007AA021503); and the 111 Project from Ministry of Education of China (B07049)
Abstract:[Objective] To Construct of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and express of vitreoscilla hemoglobin.[Methods] Intergeneric genetic transfer system was based upon plasmid pSET152 and pHZ1358 from Donor E.coli ET12567(pUZ8002) to Streptomyces venezuelae var. Qinlingensis [Results] Our data showed that Intergeneric genetic transfer system was demonstrated and optimized. Integrating plasmid pJD100 containing Perm Eand construction gene of vhb, constructed by SOE-PCR, was transformed to ET12567(pUZ8002), and then transfered to Streptomyces venezuelae var. Qinlingensis by conjugation. The integrating expression of vhb gene in Streptomyces venezuelae var. Qinlingensis was verified by PCR and CO binding diference spectrum. [Couclusion] In summary, intergeneric genetic transfer system was demonstrated and optimized, and this is the first report to express vhb gene in Streptomyces venezuelae var. Qinlingensis.
Feng Gao, Bin Wang, Taichun Chen, Zhijun Wang, Derong An. Construction of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and the expression of vitreoscilla hemoglobin. [J]. Acta Microbiologica Sinica, 2010, 50(3): 406-410
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