Multilocus sequence typing of animal source Camplobacter jejuni in east China

Abstract:

Abstract:[Objective] To characterize the genes of Campylobacter jejuni isolates and to genotype the strains from chicken, cow, duck, pig and red-crowned Crane during 2006 to 2008 by multilocus sequence typing (MLST). [Methods] To choose 7 pairs Campylobacter jejuni home-keeping gene, gltA, aspA, glnA, glyA, pgm, 、tkt and uncA as target gene, PCR was applied to detect 42 samples in East China between 2006-2008. Sequencing, analysis and comparation of the genes were done. [Result] Typing the 42 isolates by MLST and finding 24 new sequence typing types. [Conclusion] MLST is a useful method to evuluate Campylobacter jejuni genes and gene evolution.

Key words:Keywords: Campylobacter jejuni; Multilocus sequence typing (MLST); gene typing