Baofeng Chai
Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, ChinaYanqin Hao
Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, ChinaQuan Shen
Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, ChinaAihua Liang
Key Laboratory of Chemical Biology and Molecular Engineering of The Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006, ChinaSupported by grants from the National Natural Scientific Foundation of China (30770294, 30940043) and the Natural Scientific foundation of Shanxi (2009011040-1)
Abstract: The protein biosynthesis in cells is an open process cooperatively regulated by many protein factors and enzymes, which form a complicated protein network and signal transduction pathway for mRNA metabolism and protein translation. [Objective] To provide a platform for studying on the function of proteins involving in process of translation termination and the relationship among these proteins in ciliates. [Methods] We constructed the cDNA library of protozoan ciliates Euplotes octocarinatus strictly following the procedure of BD MatchmakerTM Library Construction & Screening kit from Clontech. [Results] We obtained a cDNA library of ciliates Euplotes, the titer of which was -2.437×107cfu/mL, which could suffice for functional gene screen. By using the class II polypeptide release factor (eRF3) as bait protein, we obtained some putative genes, including a partial cDNA putatively encoding RNA helicase. [Conclusion] This library will provide a convenient platform for identifying the functional genes in ciliates.
Baofeng Chai, Yanqin Hao, Cui Li, Quan Shen, Aihua Liang. Construction of cDNA library of protozoan ciliates Euplotes octocarinatus. [J]. Acta Microbiologica Sinica, 2010, 50(3): 317-321
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