Differential expression proteins of Bacillus anthracis cultured in rabbit’s intestinal tract model

Abstract:

Abstract：[Objective] The rabbit’s intestinal tract model was constructed to analyze the different expression proteins of Bacillus anthracis in vitro and in vivo. [Methods] We used the rabbit’s intestinal tract model to culture the Bacillus anthracis, using different methods to extract the extracellular proteins, cell wall proteins and whole cellular proteins from the in vivo and in vitro samples. Those proteins were separated by two－dimensional gel electrophoresis and analyzed by mass spectrum. [Results] We had been distinguished about 144 differential expression protein spots between in vivo and in vitro samples, and 124 spots were identified, including 19 supernatant proteins, 29 cell wall proteins and 78 whole cellular proteins. [Conclusion] Those proteins which related to the anabolic metabolism were down-regulated in vivo, including threonyl－tRNA synthetase, prolyl-tRNA synthetase, long-chain-fatty-acid-CoA ligase. There were also many up-regulated proteins in vivo, such as DnaK, SodA and S-layer proteins.

Key words:Keywords: Bacillus anthracis vaccine strain A16R; the rabbit’s intestinal tract model; two-dimensional gel electrophoresis