Jianhua Xu
College of Life Science and Biotechnology of XinJiang University, Urumqi 830046, ChinaHongmei Yang
Institute of Microbiology, XinJiang Academy of Agriculture Science, Urumqi 830091, ChinaJun Zeng
College of Life Science and Biotechnology of XinJiang University, Urumqi 830046, ChinaZhi Li
College of Life Science and Biotechnology of XinJiang University, Urumqi 830046, ChinaKai Lou
Institute of Microbiology, XinJiang Academy of Agriculture Science, Urumqi 830091, ChinaSupported by the Key Project of National Programs for Fundamental Research and Development(2008CB417214) and the Open Project of the Key Lab of Microorganisms in Xinjiang Specific Environment(XJYS0203-2007-04)
Abstract：[Objective] In order to investigate bacteria diversity in a hot gas spring soil of MaNasi county, Xinjiang. [Methods] Total DNA were directly extracted from the soil of a hot gas spring from Xinjiang.16S rDNA were amplified directly from purified DNA by PCR with universally bacteria-specific rDNA primers and cloned. Positive clones were identified by restriction fragment length polymorphism(RFLP), and unique rDNA types clones were sequenced, analysed and then constructed phylogenetic tree. [Results] Twenty-nine operational taxonomic units (OTU) from 170 positive clones were found belonging to 6 phyla: Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, Planctomycetes, Actinobacteria. Firmicutes (71%) was the absolutely dominant components of the soil bacterial community. 14 sequences (about 1.5 kb) from 29 OTUs showed less affiliation with known taxa(<97% sequence similarity).[Conclusion] Soil bacterial diversity is low in the hot gas spring soil, but exit a large number of new unknown taxon in this environment.
Jianhua Xu, Hongmei Yang, Jun Zeng, Zhi Li, Kai Lou. Bacterial diversity in a hot gas spring soil of MaNasi County, Xinjiang by culture-independent approach. [J]. Acta Microbiologica Sinica, 2010, 50(5): 568-573
Copy
WeChat ID 
Mobile Terminal Acta Microbiologica Sinica ® 2025 All Rights Reserved
