Flocculating activity of Penicillium purpurogenum EL-02

Abstract:

Abstract: [Objective] To isolate, identify and characterize a fungus with flocculating activity. [Methods] We used gradient dilution, plaque distribution and 18S rDNA analysis to isolate and identify a fungus with flocculating activity. We used high-speed centrifugation, ultrasonication and qualitative test to determine the nature of flocculating active substances. [Results] We isolated a flocculating active fungi and identified it as Penicillium purpurogenum. Ultrasonication test confirmed that the flocculating activity was primarily in the fermentation supernatant. We explored the flocculating activity curve and found that 4 days was the optimum fermentation time for accumulating flocculating active substances. The strain flocculating activity remains unchanged when pH varied from 2 to 11 and temperature varied from -70℃ to 100℃.We finally identified the flocculating active substances as saccharides. [Conclusion] We isolated a flocculating active strain P. purpurogenum EL-02, and identified its flocculating active products as saccharides.

Key words:Keywords: flocculating activity; fungi isolation and identification; active substances; Penicillium purpurogenum