Isolation and identification of a polyhydroxyalkanoate producing strain

Abstract:

Abstract: [Objective] We screened and isolated polyhydroxyalkanoate producing bacteria. [Methods] The strains were isolated from sludge from a beer brewery and screened by Sudan black B staining method. The isolated strains were identified according to their morphological features, physiological and biochemical analysis as well as 16S rRNA gene sequence analysis. The product extracted with hot chloroform from the isolated strain HG-B-1 was confirmed by Fourier transform infrared spectra. [Results] We isolated a bacterium, HG-B-1, from sludge collected from a beer brewery in Guangdong province, China. The yield of polyhydroxyalkanoates was 23.4％(w/w) based on dried weight of the bacterium cells when HG-B-1 grew in a medium containing saccharose. We analyzed 16S rRNA nucleotide sequence, and ascertained the phylogenetic position of the strain. [Conclusion] Strain HG-B-1 with PHAs biosynthesis ability was identified as Stenotrophomonas maltophlia.

Key words:Keywords：polyhydroxyalkanoates (PHAs); screening; identification; Stenotrophomonas maltophlia