Liwei Zhao
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaChaohui Hu
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaRuihong Xuan
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaYangmin Liu
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaQingyi Zhu
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaJuan Wang
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaXiaoyong Zhan
Guangzhou Kingmed Center for Clinical Laboratory, Guangzhou 510330, ChinaSupported by the National Standardization Committee (20081021-T-361) and the Ministry of Science and Technology of the P. R. China (2008ZX10004-006)
Abstract: [Objective] To evaluate polymerase chain reaction (PCR) combined with enzymatic digestion for identification of Legionella, and distribution of Legionella in environmental water systems in Guangzhou. [Methods] Forty-four water samples collected in Guangzhou were cultivated for Legionella were identified by PCR-enzymatic digestion, 16S rDNA and mip gene sequencing analysis. [Results] Sixty-six strains of Legionella pneumophila and 46 Non-L. pneumophila were identified by PCR-enzymatic digestion and sequencing analysis. Forty-six strains of Non-L. pneumophila included 20 strains of L. feeilei, 17 L. gormanii, 7 L. oakridgensis and 2 L. longbeachae. [Conclusion] PCR combined with enzymatic digestion is a simple, rapid, and specific method for the identification of Legionella. L. pneumophila was distributed widely, followed by L. feeilei, L. gormanii, L. oakridgensis and L. longbeachae, in environmental water in Guangzhou area.
Liwei Zhao, Chaohui Hu, Ruihong Xuan, Yangmin Liu, Qingyi Zhu, Juan Wang, Xiaoyong Zhan. Identification of Legionella isolates from environmental water by using PCR combined with enzymatic digestion analysis. [J]. Acta Microbiologica Sinica, 2010, 50(11): 1532-1536
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