Weibin Song
Institute of Biochemistry, Zhejiang University, Hangzhou 310058, ChinaYanqing Lin
Institute of Biochemistry, Zhejiang University, Hangzhou 310058, ChinaHaiyan Hu
Hangzhou Bioking Biochemical Engineering Co., Ltd., Hangzhou 311106, ChinaZhipeng Xie
Institute of Biochemistry, Zhejiang University, Hangzhou 310058, ChinaJianguo Zhang
Institute of Biochemistry, Zhejiang University, Hangzhou 310058, ChinaSupported by the National Key Infection Disease Project (2008ZX10003-006, 2008ZX10003-001)
Abstract: [Objective] To isolate a new osmophilic yeast for producing D-arabitol and research its optimal fermentation conditions for highest yield of D-arabitol from glucose. [Methods] The isolated strain was characterized by electron microscopy, Biolog (GN) test, G+C content measurement and 26S rDNA D1/D2 domain sequences analysis. The purified fermentation product was identified by IR, 1H-NMR, 13C-NMR, MS and optical rotation analysis. Then the fermentation conditions for D-arabitol production were optimized. [Results] A new osmophilic yeast was isolated and identified as Candida sp. H2. Through the single factor experiment, the optimum conditions of 250 g/L glucose, 10 g/L yeast extract, initial pH 6.0, 35 °C of culture temperature, 200 rpm of agitation, 200 mL medium in a 1000-mL flask of broth content, 1% (v/v) of inoculum size, 96 h of fermentation time were achieved. Based on the conditions above, weight yield of 35% (86.55 g D-arabitol from 250 g glucose) was obtained and 10% higher than the conditions not optimized. [Conclusions] Candida sp. H2 was a novel strain for producing D-arabitol and valuable for further study.
Weibin Song, Yanqing Lin, Haiyan Hu, Zhipeng Xie, Jianguo Zhang. Isolation and identification of a novel Candida sp. H2 producing D-arabitol and optimization of D-arabitol production. [J]. Acta Microbiologica Sinica, 2011, 51(3): 332-339
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