Construction of a recombination plasmid labelled with green fluorescence and its application in Clonostachys rosea

Abstract:

Abstract:［Objective］We constructed a recombinant eukaryotic expression vector harboring green fluorescent protein (GFP) and the hygromycin resistance gene hph，and observed its expression in Clonostachys rosea．［Methods］We used PCR， enzyme digestion， phosphorylation， ligation and transformation to construct the plasmid． Using protoplast preparation and transformation technologies，we expressed the plasmid in the fungi C．rosea．［Results］We created the eukaryotic expression vector，transformed it into C．rosea and observed green fluorescence with fluorescence microscope．［Conclusion］The successful construction of the pANGH3 recombinant plasmid and its expression in C． rosea establishes a new model for studying fungal infection mechanisms．