Screening，identification，and promoting effect of phosphate-solubilizing bacteria in rhizosphere of Taxus chinensis var.mairei

Abstract:

Abstract:［Objective］ Phosphate-solubilizing bacteria (PSB) were isolated，screened and identified from the rhizosphere of Taxus chinensis var． mairei，and growth-promoting effects on T．chinensis var． mairei by high effective PSB were determined．［Methods］ By using selective culture media，PSB were isolated from rhizospheric soil，the high effective PSB was further screened using NBRI-BPB medium，and the molybdenum-antimony anti-spectrophotometric method was applied to determine the phosphate-dissolving ability of the high effective PSB after four days fermentation in NBRIP medium．Bacteria were identified by the Biolog system combined with 16S rDNA gene sequence analysis and morphological，physiological and biochemical characteristics． The inoculation test in potted seedlings was carried out under the greenhouse．［Conclusion］ Four strains of high effective PSB were screened and identified as Pseudomonas fluorescens，Bacillus cereus，Sinorhizobium meliloti and Bacillus licheniformis，respectively． These strains had significant effects on improving the growth of the seedlings of T．chinensis var．mairei．