Identification of transcriptional regulatory sequences of yigP gene in Escherichia coli

Abstract:

Abstract: ［Objective］ We investigated the promoter activity of yigP gene and analyzed its transcriptional regulatory sequence．［Methods］We cloned promoter fragment into promoter probe plasmid which has a reporter gene lacZ，thus the promoter activity could be measured by detecting β-galactosidase activity． Then，the promoter region was minimized by cloning different truncated promoter fragments into promoter probe plasmid． Using site-directed mutagenesis technology，we introduced site mutations in some key sequences and investigated their effects on promoter activity．［Results］ The promoter region of yigP gene was determined，and also the -10 region and -35 region were identified． Meanwhile，a negative regulatory sequence just upstream of yigP promoter was discovered，and our results show that these sequences have important influence on the transcriptional regulation．［Conclusion］ We identified the transcriptional regulatory sequences of yigP gene，and this facilitated our understanding of gene transcription．