Using reporter gene to compare infection efficiency of HIV-1 pseudovirus to suspended and adherent cells
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Supported by the National Natural Science foundation of China (31160191)

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    Abstract:

    Abstract:[Objective]In order to compare the infection of HIV-1 pseudovirus to suspended and adherent cells,Tzmbl cells containing β-gal ( β-galactosidase ) reporter gene were used here to do the analysis. [Methods] HIV-1 pseudoviruses were generated by co-transfection of 293T cells with the plasmid pNL43R-E- and HIV envelope expressing plasmid. Supernatant of co-transfected 293T cells was collected and used to infect Tzmbl cells with or without trypsin treatment. Forty-eight hours after infection,β-gal positive Tzmbl cells and virus infection were determined using X-gal staining and β-glo (β-galactosidase) assay. [Results]The efficiency of HIV pseudoviruses infection of suspended Tzmbl cell was higher than that of adherent cells and the increase of infection correlated with the pseudoviral subtype.[Conclusion]This study may provide a useful method for HIV biological study and neutralization assays using a singleround replicative pseudovirus in the future.

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Yan Su, Lingling Su, Tao Yin, Baojiang Zhang. Using reporter gene to compare infection efficiency of HIV-1 pseudovirus to suspended and adherent cells. [J]. Acta Microbiologica Sinica, 2012, 52(7): 921-926

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History
  • Received:January 04,2012
  • Revised:March 06,2012
  • Online: July 09,2012
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