PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein

Abstract:

Abstract:［Objective］To identify the interaction between influenza A virus PB1-F2 and human modulator of apoptosis 1 (MOAP-1)．［Methods］The recombinant plasmid pACT2-MOAP-1 was constructed and co-transformed into yeast AHl09 with pGBKT7-PB1-F2． The growth of the co-transformants on quadruple dropout medium and β-galactosidase activity of the reporter gene were tested． We further confirmed the interaction of cellular protein MOAP-1 and PB1-F2 by glutathione S-transferase(GST)pull-down and co-immunoprecipitation (Co-IP) assays． In addition，we investigated the effect of PB1-F2 on MOAP-1 protein level by Western blot．［Results］The results of yeast two-hybrid assay showed that MOAP-1 specifically interacted with PB1-F2 in yeast cells． Furthermore，the binding of MOAP-1 with PB1-F2 was demonstrated by glutathione S-transferase pull-down and Co-IP assays． PB1-F2 could upregulate exogenous MOAP-1 protein level．［Conclusion］These results suggested that influenza virus PB1-F2 interacted with MOAP-1 and it might be involved in the regulation of cell growth and apoptosis via association with MOAP-1．