PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein
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Supported by the National Natural Science Foundation of China(30800824) and the National Key Basic Research Program of China (973 Program) (2012CB518900)

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    Abstract:

    Abstract:[Objective]To identify the interaction between influenza A virus PB1-F2 and human modulator of apoptosis 1 (MOAP-1).[Methods]The recombinant plasmid pACT2-MOAP-1 was constructed and co-transformed into yeast AHl09 with pGBKT7-PB1-F2. The growth of the co-transformants on quadruple dropout medium and β-galactosidase activity of the reporter gene were tested. We further confirmed the interaction of cellular protein MOAP-1 and PB1-F2 by glutathione S-transferase(GST)pull-down and co-immunoprecipitation (Co-IP) assays. In addition,we investigated the effect of PB1-F2 on MOAP-1 protein level by Western blot.[Results]The results of yeast two-hybrid assay showed that MOAP-1 specifically interacted with PB1-F2 in yeast cells. Furthermore,the binding of MOAP-1 with PB1-F2 was demonstrated by glutathione S-transferase pull-down and Co-IP assays. PB1-F2 could upregulate exogenous MOAP-1 protein level.[Conclusion]These results suggested that influenza virus PB1-F2 interacted with MOAP-1 and it might be involved in the regulation of cell growth and apoptosis via association with MOAP-1.

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Yuming Cui, Peili Hou, Maolin Zhang, Ming Duan, Zhenhong Guan. PB1-F2 protein of influenza A virus interacts with human MOAP-1 protein. [J]. Acta Microbiologica Sinica, 2012, 52(9): 1122-1128

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History
  • Received:May 15,2012
  • Revised:June 05,2012
  • Online: September 12,2012
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