Wenjie Gu
Soil and Fertilizer Institute，Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region，Ministry of Agriculture，Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation Guangzhou 510640，ChinaYouquan Xu
College of Life Science，Northeast Agricultural University，Harbin 150030，ChinaPeizhi Xu
Soil and Fertilizer Institute，Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region，Ministry of Agriculture，Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation Guangzhou 510640，ChinaKaizhi Xie
Soil and Fertilizer Institute，Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region，Ministry of Agriculture，Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation Guangzhou 510640，ChinaYusheng Lu
Soil and Fertilizer Institute，Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region，Ministry of Agriculture，Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation Guangzhou 510640，ChinaShuanhu Tang
Soil and Fertilizer Institute，Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region，Ministry of Agriculture，Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation Guangzhou 510640，ChinaFabao Zhang
Soil and Fertilizer Institute，Guangdong Academy of Agricultural Sciences Key Laboratory of Plant Nutrition and Fertilizer in South Region，Ministry of Agriculture，Guangdong Key Laboratory of Nutrient Cycling and Farmland Conservation Guangzhou 510640，ChinaSupported by the Cooperation Project in production，Education and Reaserch from Guangdong Province Department of Education(2011B090400277) and by the Special Funds of Scientific Reaserch for Public Welfare Industry(Agriculture) (201003016)
Abstract:［Objective］The aim of this study was to screen hemicellulose degrading microorganisms．［Methods］ The methods used to screen the effective strains included hydrolysis spot diameter measurement of hemicellulose plate and extracellular enzyme activity． The methods used to identify the strains included culture characteristics，morphological，physiological-biochemical characteristics and molecular biological methods． ［Results］ We isolated 4 actinomycetes (NA9，NA10，NA12 and NA13)，2 fungi (NF1 and NF7) with hemicellulose degrading ability and no antagonistic effect among them． The hemicellulose degrading activity of 4 actinomyces (NA9，NA10，NA12 and NA13) was 217.6，229.8，221.1 and 211.8 U/mL． The hemicellulose degrading activity of 2 fungi (NF1 and NF7) was 217.7 and 244.2 U/mL．The hemicellulose degrading activity of complex microbial system was 299. 0 U/mL．NA9，NA10，NA12 and NA13 were Streptomyces costaricanus; NF1 was Aspergillus candidus and NF7 was Tarlaromyces flavus． ［Conclusion］the 4 actinomyces and 2 fungi screened have high hemicelluloses enzyme activity． These strains have good application value and more research value.
Wenjie Gu, Youquan Xu, Peizhi Xu, Kaizhi Xie, Yusheng Lu, Shuanhu Tang, Fabao Zhang. Screening and identification of hemicellulose degrading microorganisms in acid soil. [J]. Acta Microbiologica Sinica, 2012, 52(10): 1251-1259
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