Effects of ultra-high pressure on membrane components in Vibrio parahaemolyticus

Abstract:

Abstract:［Objective］We observed the changes in cell membrane of Vibrio parahaemolyticus which is a prevalent foodborne pathogen by high pressure treatments． ［Methods］Pressure-resistant mutant strains of V． parahaemolyticus were selected with repeated hydrostatic pressures treatment of 80－250MPa from a pressure-sensitive V． parahaemolyticus (ZJGSMC001)．The changes of soluble cell membrane protein，fatty acid profiles and the Na + K + ATPase activity in the pressure-resistant strains and its pressure-sensitive parent strain were determined． ［Results］The pressure-resistant strains had more soluble cell membrane protein of 36 KDa． The Na + K + ATPase of the pressure-resistant strains were 83. 3% more active than the parent strain． The proportion of unsaturated fatty acids in cell membranes was 54. 23% comparing to 51. 57 % (P＜0.05) in the pressure-sensitive strain． ［Conclusion］The pressure-resistant strains may have survived pressure treatments through expressing more low-molecular soluble cell membrane proteins，enhancing the activity of Na+K+ATPase，and increasing the content of unsaturated fatty acids in cell membrane．

Key words:Keywords: Vibrio parahaemolyticus，cell membrane composition，pressure-resistant mechanism