Adenovirus-mediated canine interferon-γ expression and its antiviral activity against canine parvovirus

Abstract:

Abstract:［Objective］ To construct recombinant adenovirus containing canine interferon-γ (cIFN-γ) gene and to investigate its antiviral activity against canine parvovirus in Madin-Darby canine kidney cells (MDCK)．［Methods］The cIFN-γ gene was inserted into adenovirus shuttle plasmid to construct pShuttle3-cIFN-γ expression vector，from which the cIFN-γ expression cassette was transferred into the adenovirus genomic plasmid pAdeno-X by specific restriction sites to generate recombinant adenovirus genomic plasmid pAd-cIFN-γ． The pAd-cIFN-γ plasmid was linearized by digestion and transfected into human embryonic kidney ( HEK) 293T cells to generate the replication-defective cIFN-γ recombinant adenovirus (Ad-cIFN-γ) ． To analyze its anti-canine parvovirus activity，the MDCK cells were pre-infected by Ad-cIFN-γrecombinant adenovirus，and then infected by canine parvovirus． The antiviral activity of the Ad-cIFN-γ recombinant adenovirus against parvovirus was analyzed． ［Results］ The recombinant adenovirus containing cIFN-γ gene was constructed by the ligation method． The recombinant adenovirus could mediates recombinant cIFN-γ secretory expression in MDCK cells． The Ad-cIFN-γ recombinant adenovirus could significantly inhibit canine parvovirus replication in MDCK cells pre-infected with the recombinant adenovirus． These results indicate that the Ad-cIFN-γ recombinant adenovirus has the potent antiviral activity against canine parvovirus． ［Conclusion］ The Ad-cIFN-γ recombinant adenovirus was successfully constructed by the ligation method and possessed a powerful antiviral activity against canine parvovirus．