Activity of the SrfAC-A domain from Bacillus subtilis fmbj

Abstract:

Abstract:［Objective］ We studied the A domain of surfactin synthase in vitro to obtain new surfactin analogues．［Methods］We cloned the srfAC-A gene from Bacillus subtilis fmbj by PCR，and constructed a recombinant expression vector named pET-23a-srfAC-A． Furthermore，the SrfAC-A domain was expressed in E．coli BL21 (DE3) and purified by Ni-NTA agarose column． Then the activity of srfAC-A domain was detected． ［Results］The srfAC-A domain had specificity towards Ile，but almost no activity to other amino acids．［Conclusion］The independent A domain from surfactin synthase had selectivity to specific amino acids in vitro．