Distribution of endophytic fungi in Oxytropis glabra DC

Abstract:

Abstract:［Objective］ Endophytic fungi were detected and isolated from the stems，leaves，petioles and seeds of Oxytropis glabra DC sampled from Alashan of Inner Mongolia to investigate the infection rate and species distribution in different tissues．［Methods］ The endophytic fungi infection rate and distribution of species in different tissues were investigated by making temporary slides，staining，isolation and identification．［Results］Endophytic fungi were detected and observed from all parts (stems，leaves，petioles and seeds) of the plant by temporary slides staining．A total of 79 isolates were cultivated from 4 different tissues by common separation methods，which belonged to 10 genera after identification． The infection rate and separation rate were seeds＞leaves＞stems＞petioles． Undifilum oxytropis，Embellisia sp． L12 and Fusarium equiseti were the dominant species in this plant with the relative isolate frequency 77.32%，64.00% and 50.00%，respectively． ［Conclusion］Endophytic fungi were commonly found in the all parts of Oxytropis glabra DC． There was an obviously difference in quantity，species and distribution of the endophytic fungi between different parts of plant． Seeds and leaves were the most vulnerable to infection and colonization by the endophytic fungi．