Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp．40027

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Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp．40027
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                        Xumei GengXumei Geng
Key Lab for Biotechnology of the State Ethnic Affairs Commission，College of Life Science，South-Central University for Nationalities，Wuhan 430074，China
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Xin ChenXin Chen
Key Lab for Biotechnology of the State Ethnic Affairs Commission，College of Life Science，South-Central University for Nationalities，Wuhan 430074，China
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Xiaotong YangXiaotong Yang
Key Lab for Biotechnology of the State Ethnic Affairs Commission，College of Life Science，South-Central University for Nationalities，Wuhan 430074，China
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Jia GuoJia Guo
Key Lab for Biotechnology of the State Ethnic Affairs Commission，College of Life Science，South-Central University for Nationalities，Wuhan 430074，China
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Guifa ZhaiGuifa Zhai
Key Lab for Biotechnology of the State Ethnic Affairs Commission，College of Life Science，South-Central University for Nationalities，Wuhan 430074，China
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Xiaohua LiXiaohua Li
Key Lab for Biotechnology of the State Ethnic Affairs Commission，College of Life Science，South-Central University for Nationalities，Wuhan 430074，China
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Fund Project:Supported by the National Natural Science Foundation of China (31070087，30570046)，by the Natural Science Foundation of Hubei province (2011CDA079，2008CDB076) and by the National College Students Innovative Entrepreneurial Training Program (GCX12012)

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Abstract:

Abstract:Micoromonospora sp．40027，the producer of fortimicin A，harbors two plasmids pJTU101 and pJTU112．［Objective］Cloning and sequencing of replication region of pJTU112，analyzing replication region sequence of pJTU112．［Methods］Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp． LXH20．The replication region of pJTU112 was identified．［Results］The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment．DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames．The pJTU112.1 and pJTU112.2 were related to plasmid conjugation，pJTU112.3，pJTU112.4 and pJTU112. 5 were related to plasmid conjugation．［Conclusion］ The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment．

Key words:Keywords: Micromonospora sp．40027，plasmid pJTU112，replication region，cloning

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Xumei Geng, Xin Chen, Xiaotong Yang, Jia Guo, Guifa Zhai, Xiaohua Li. Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp．40027. [J]. Acta Microbiologica Sinica, 2013, 53(6): 623-627

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Received:November 15,2012
Revised:January 09,2013
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Online: June 05,2013
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