Yanling Wang
State Key Laboratory of Mycology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaPengjie Hu
State Key Laboratory of Mycology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaErwei Li
State Key Laboratory of Mycology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaXingzhong Liu
State Key Laboratory of Mycology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaYongsheng Che
Beijing Institute of Pharmacology ＆ Toxicology，Beijing 100850，ChinaGang Liu
State Key Laboratory of Mycology，Institute of Microbiology，Chinese Academy of Sciences，Beijing 100101，ChinaSupported by the Project of Knowledge Innovation of Chinese Academy of Sciences (KSCX2-EW-J-6)
Abstract:［Objective］ To construct a transformation system in Gliocladium sp．6.102，a Cordyceps-colonizing fungus producing a variety of epipolythiodioxopiperazine (ETP) compounds with drug potentials.［Methods］ Agrobacterium tumefaciens-mediated transformation (ATMT) was used to transform Gliocladium sp. 6. 102. The factors of bacterial cell concentration，co-cultivation time，pH and acetosyringone concentration were optimized．［Results］A total of 50－100 transformants per 106 fungal conidia was obtained via the optimal ATMT method．The genes encoding hygromycin B phosphotase and enhanced green fluorescent protein (EGFP) were transferred into Gliocladium sp. by the optimal ATMT method. The marker genes were successfully expressed and stably maintained in the transgenic fungus.［Conclusion］A transformation system was established for Gliocladium sp. 6. 102 and this system may be useful to identify ETP biosynthetic genes in Gliocladium．
Yanling Wang, Pengjie Hu, Erwei Li, Xingzhong Liu, Yongsheng Che, Gang Liu. Genetic transformation of the fungus Gliocladium sp．mediated by Agrobacterium tumefaciens. [J]. Acta Microbiologica Sinica, 2013, 53(11): 1233-1239
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