Screening and identification of Bacillus pumilus producing double active protein of anticoagulation and thrombolysis

Abstract:

Abstract:［Objective］The aim of this study was to screen bacteria that can produce antithrombotic．［Methods］ We screened the target bacteria on VY/4 plate and casein plate from more than 20 samples such as water，soil，rabbit manure，sheep manure and deadwood．We detected the antithrombotic activity by fibrin plate and fibrin tube．We identified the target bacteria by morphological characteristics，physical and chemical properties and 16S DNA sequence homology.［Results］We obtained 5 strains that can produce antithrombotic．We found that the extracellular protein of strain LDS33 shows both stronger fibrinolytic activity and stronger anticoagulation activity．According to the morphology，physiochemical properties，16S DNA sequencing and phylogenetic tree，strain LDS33 is identified as Bacillus pumilus．［Conclusion］Bacillus pumilus LDS33 can produce highly active anticoagulation and thrombolysis double active protein.