Immunogenicity of co-expressed p30-gp90 against Reticuloendotheliosis virus

Abstract:

Abstract:［Objective］ To study the immunogenicity of co-expression of p30，one of a group specific antigens，and glycoprotein gp90 genes of Reticuloendotheliosis virus (REV)．［Methods］p30 and gp90 genes were amplified with the template of plasmid pPB101 containing the whole sequence of spleen necrosis virus(SNV)，and cloned into pET-28a(+) vector．The positive clone pET-p30-gp90 was identified by enzyme analysis and sequencing．The co-expressed protein p30-gp90 was confirmed by SDS-PAGE and Western blot analysis． After quantitation，the purified protein p30-gp90 was immunized to Balb/c mice three times to prepare the p30-gp90 specific anti-serum．Afterwards，we detected the REV infected Chick Embryo Fibroblasts (CEF) by Immunofluorescence assay (IFA) with the prepared anti-serum．［Results］The p30-gp90 was co-expressed efficiently by SDS-PAGE and Western blot analysis．The anti-serum of p30-gp90 reacts with REV infected CEF in IFA．［Conclusion］The expressed protein of p30-gp90 keeps good immunogenicity．