Effects of transgenic Bt + CpTI cotton on rhizosphere bacteria and ammonia oxidizing bacteria population

Abstract:

Abstract:The effect of transgenic cotton on the rhizosphere bacteria can be important to the risk assessment for the genetically modified crops.［Objective］ We studied the rhizosphere microbial community with cultivating genetically modified cotton．［Methods］The effects of transgenic Bt + CpTI Cotton (SGK321) and its receptor cotton (SY321) on rhizosphere total bacteria and ammonia oxidizing bacteria population size were studied by using droplet digital PCR．We collected rhizosphere soil before cotton planting and along with the cotton growth stage (squaring stage，flowering stage，belling stage and boll opening stage)．［Results］There was no significant change on the total bacterial population between the transgenic cotton and the receptor cotton along with the growth stage．However，the abundance of ammonia oxidizing bacteria (AOB) in both type of cottons showed significant difference between different growth stages，and the variation tendency was different．In squaring stage，the numbers of AOB in rhizosphere of SY321 and SGK321 increased 4 and 2 times，respectively． In flowering stage，AOB number in rhizosphere of SY321 significantly decreased to be 5.96×105 copies/g dry soil，however，that of SGK321 increased to be 1.25×106 copies/g dry soil．In belling stage，AOB number of SY321 greatly increased to be 1.49×106 copies/g dry soil，but no significant change was observed for AOB number of SGK321．In boll opening stage，both AOB number of SY321 and SGK321 clearly decreased and they were significantly different from each other．Compared to the non-genetically modified cotton，the change in abundance of ammonia oxidizing bacteria was slightly smooth in the transgenic cotton，［Conclusions］Not only the cotton growth stage but also the cotton type caused this difference． The transgenic cotton can slow down the speed of ammonia transformation through impacting the number of AOB，which is advantageous for plant growth．

Key words:Keywords: Droplet digital PCR，ammonia oxidizing bacteria，transgenic Bt + CpTI cotton，Shiyuan321，amoA，16S rRNA