miR432* regulate the replication of Coxsackievirus A16 in rhabdomyosarcoma cells

Abstract:

Abstract:［Objective］MicroRNAs (miRNAs) play an important role in infection and replication of virus in host cells．In this study，we examined miＲNAs’effects on the replication of Coxsackievirus A16 ( CA16) in rhabdomyosarcoma cells.［Methods］We constructed target gene of miRNAs screening system． We used 3' untranslated region (UTR) dual luciferase reporter analysis to identify putative miRNA targets in the CA16 virus genome．First，12 segments of CA16 virus genome were inserted to the pMIＲ vector and the luciferase expression were assayed to identify the target gene of putative miRNA．The reporter gene expression of the cells transfected with the vector containing 5'-UTR was significantly downregulated．Then，using online analysis programs we screened the miRNAs that may target to 5'-UTR．Furthermore，Western blot and real-time PCR test were used to study the effect of miRNAs on viral replication.［Results］The study showed that miR432* could stimulate the replication of CA16 virus．On the contrary，miR432* inhibitor could suppress CA16 virus replication.［Conclusion ］Cellular miRNAs could regulate the replication of CA16 virus in host cells．Our findings support the notion that the cellular miRNAs play an important role in the host and virus infection．