Construction and characterization of R169H mutant of aspartokinase from Corynebacterium pekinense

Abstract:

Abstract:［Objective］Increasing the activity of aspartokinase (AK) from Corynebacterium pekinense.［Methods］The gene of AK was constructed and mutated by site-specific mutagenesis．The mutational recombinant plasmid was heterologously expressed in Escherichia coli BL21．The mutational AK was purified by Ni2 + -NTA column after ultrasonicating of the recombinant bacteria，and then identified by SDS-PAGE and Western blot．We compared the kinetic difference between R169H AK and WT AK by determining the enzymatic activities．Some other characteristics of R169H AK and WTAK were also studied.［Results］The mutant R169H was successfully constructed．The molecular weight of AK was 48kDa．Vmax of R169H AK was 226.3 U/mg·s－1，which was 2.3 times higher than that of WT AK．The optimum reaction temperature of R169H AK was 26℃，the same as that of WT AK．The optimum reaction pH of R169H AK was 9.0，slightly higher than that of WT AK．The half-life period of R169H AK under optimum temperature and pH were 5.5h，much higher than that of WT AK．Lysine，threonine and methionine had an active effect on the activity of R169H AK when they were in low concentration.［Conclusion］ The hydrogen bond between R169 and E92 was broken down in R169H AK，which could affect the degree of polymerization and further lowered the affinity of mutant AK with substrates and then decreased the inhibition inducing by the metabolites. Thus，the Vmax of mutant AK from R169H had increased by 2.3 times compared with that of WT AK.