Yi Wang
Conservation of Rare，Endangered ＆ Endemic Forest Plants，Public Key Laboratory of the State Forestry Administration;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants; Yunnan Academy of Forestry，Kunming 650204，Yunnan Province，ChinaXu Zhou
Conservation of Rare，Endangered ＆ Endemic Forest Plants，Public Key Laboratory of the State Forestry Administration;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants; Yunnan Academy of Forestry，Kunming 650204，Yunnan Province，ChinaJae-Seoun Hur
Korean Lichen Research Institute，Sunchon National University，Sunchon 540742，Republic of KoreaJuan Wang
Conservation of Rare，Endangered ＆ Endemic Forest Plants，Public Key Laboratory of the State Forestry Administration;Yunnan Provincial Key Laboratory of Cultivation and Exploitation of Forest Plants; Yunnan Academy of Forestry，Kunming 650204，Yunnan Province，ChinaSupported by the Middle Aged Academic and Technical Leader Project of Yunnan Province (2010CI016)
Abstract:［Objective］To isolate polyketide synthase (PKS) gene from medicinal Usnea longissima lichen forming fungi，and identify the function of obtained PKS.［Methods］We used Usnea． longissima lichen forming fungi to isolate PKS gene by nested PCR using degenerate primers and screening a Fosimid genomic library．MEGA 4.0. 2 program was used for phylogenetic analysis and RT-PCR was used to detect gene expression.［Results］ We obtained a gene cluster including non-reducing PKS (UlPKS5)，putative β-lactamase and putative dehydratase from Usnea longissima lichen forming fungi．UlPKS5 contained ketosynthase (KS)，acyl transferase (AT)，product template (PT) and acyl carrier protein (ACP) domain． Phylogenetic analysis shows that UlPKS5 belonged to non-reducing PKS group V，which involved anthraquinone biosynthesis.RT-PCＲ analyses reveal that the expression of UlPKS5 was up-regulated by sucrose (2% and 10%) and sorbitol (10%).［Conclusion］PKS(UlPKS5)，putative β-lactamase and putative dehydratase were related with anthraquinone biosynthesis in U. longissima.
Yi Wang, Xu Zhou, Jae-Seoun Hur, Juan Wang. Isolation and charcterizaiton of a polyketide synthase gene cluster from Usnea longissima. [J]. Acta Microbiologica Sinica, 2014, 54(7): 770-777
Copy
WeChat ID 
Mobile Terminal Acta Microbiologica Sinica ® 2025 All Rights Reserved
