Quantitation of intestinal Fusobacterium and butyrateproducing bacteria in patients with colorectal adenomas and colorectal cancer

Abstract:

Abstract:［Objective］To compare the abundance of 16S rRNA gene of intestinal Fusobacterium and butyrate-producing bacteria in patients with colorectal adenomas patients and colorectal cancer and to reveal the correlation between the target bacteria and the development of colorectal cancer．［Methods］Feces were collected from colorectal cancer patients (n=19)，colorectal adenomas patients (n= 12) and healthy subjects (n=19) ．Bacteria genome DNA from the fecal samples was used to quantitate the Fusobacterium，two butyrate-producing bacteria Eubacterium rectal，Faecalibacterium prausnitzii and total bacteria by real-time polymerase chain reaction． Then the variation of the target bacteria among different groups were assayed using Mann-Whitney U test．［Results］ The abundance of Fusobacterium was significantly higher in colorectal cancer patients than that in healthy subjects (P=0.000) and colorectal adenomas patients (P=0.013)，and it was significantly higher in colorectal cancer patients than that in colorectal adenomas patients (P=0.002)．F．prausnitzii was significantly lower in colorectal adenomas patients compared to healthy subjects (P=0.033)．The total bacteria count was significantly lower in the colorectal adenomas samples than that in the healthy samples (P=0.002)．There was no significantly difference of E．rectal between the three groups．［Conclusions］ The shifts in the colonic bacterial population may potentially contribute to the development of colorectal cancer．

Key words:Keywords: colorectal cancer，colorectal adenomas，Fusobacterium，butyrate-producing bacteria，real-time PCR