Function of a calcium-dependent protein kinase gene Pscamk in Puccinia striiformis f. sp. tritici

Abstract:

Abstract:［Objective］To clone calcium-dependent protein kinase gene (camk) from Puccinia striiformis f. sp. tritici (Pst) and analyze its function.［Methods］The cDNA full-length of Pscamk was isolated by using reverse transcriptional-PCR (RT-PCR)，and gene expression profile at different morphological stages was analyzed via quantitative real-time -PCR(qRT-PCR)．Pst urediospores were treated with CaMK suppressor KN-93 and germination rate was investigated．［Results］A gene cDNA full-length with 1 620 bp was obtained and designated as Pscamk．qRT-PCR analysis showed Pscamk expression was highly induced in the early stages of Pst infection and reached the maximum at 6 h post inoculation (hpi) as 20. 74-fold as that in the control (0 hpi)．With increasing of the concentration of CaMK suppressor KN-93，germination rate of Pst urediospores was gradually decreased． The germination rate was reduced to 8.02%，only 12% of the control，under 1.4 μmol/L KN-93 treatment at 10 h after incubation at 9 ℃．［Conclusion］Pscamk might play a role in germination and germ tube elongation of Pst urediospores．This study provides a basis for exploring pathogenesis of calcium signaling pathway during Pst infection．