Absorption of fumonisin B1 and B2 by Lactobacillus plantarum ZJ8

Abstract:

Abstract:［Objective］ We studied the ability of Lactobacillus plantarum ZJ8 to bind fumonisins FB1 and FB2．［Methods］The percentage of FB1 and FB2 bound by the strain was measured by HPLC after bacterial cells and FB1 and FB2 were co-incubated in MＲS media at 37℃ for 4 h.［Results］The percentage of FB1- and FB2 -binding was 89.9% and 95.0%，respectively．The FBs-binding rate of strain ZJ8 reached the maximum at pH 4．Binding of FBs was poor under alkaline conditions and high temperatures．After acid and SDS treatment，the FBs-binding rate significantly increased．The percentage of FB1- and FB2 -binding was 96.8% and 100% for the cell walls of strain ZJ8，respectively．Moreover，about 96.8% FB1 and 100% FB2 was bound to the peptidoglycan of strain ZJ8’s cell walls.［Conclusions］L．plantarum ZJ8 has potential to remove fumonisins．The peptidoglycan of cell walls from strain ZJ8 was proved to be the main site of FBs-binding．

Key words:Keywords: Lactobacillus plantarum，fumonisins FB1，fumonisins FB2，binding