Isolation and identification of bacteria with ferro-oxidase activity

Abstract:

Abstract:［Objective］We screened and isolated Ferro-oxidase producing bacteria，for adsorbing iron and manganese．［Methods］The strains producing Ferro-oxidase were isolated from three samples of water．Ferro-oxidase producing strains were screened in shake flask culture，and identified according to morphological features，physiological and biochemical analysis as well as 16S rRNA gene sequence analysis.［Results］We isolated a bacterium S9．The strain was identified as Sphaerotilus natans．This strain had strongest adsorption on iron and manganese among the strains we identified，with 29.02mg/g iron adsorption amount in water，and 66.77% adsorption rate for 4 hours’adsorption．When the adsorption time is 6 h，the adsorption amount of manganese was 34.49mg/g，and the adsorption rate was 70.68%．The optimum temperature and pH value of Ferro-oxidase were 30℃ and 7.5，respectively．Mg2+，Na+，K+ could activate Ferrooxidase，whereas Cu2+ had little impact．While Mn2+，Zn2+ could strongly inhibit Ferro-Oxidase，Pb2+，Ag+ had only modest inhibitory effect.［Conclusion］ Strain S9 had a high Ferro-oxidase activity，and has application potential in sewage treatment.