The community structure and diversity of the endophytes in American ginseng

Abstract:

Abstract:［Objective］To clarify the composition of endophytes community in American ginseng，to know the differences of the endophytes community structure of different producing area and colonization part，and to grasp the resource situation of endophytes in American ginseng of Jilin Province． ［Methods］ Endophytes were separated with tissue explant and identified with 16S rDNA and ITS sequence analysis，by combining the cultivation forms． Moreover，ecological index analysis was conducted for the endophytes separated.［Results］Endophytes in American ginseng root obtained included 13 genera and 26 species，endophytes of the stem obtained included 10 genera and 18 species，whereas endophytes of the leaves obtained included 9 genera and 16 species． Bacillus and Pseudomonas were advantageous genera in the endophytes in American ginseng，and the species contained occupied 17.6% and 14.7% of the total endophytes respectively．According to the diversity index(H')，Hunchun＞Jingyu＞Fusong＞Ji’an＞Changbai; according to the evenness index(J)，Hunchun＞Ji’an＞Jingyu＞Fusong＞Changbai; according to the dominance index (D)，Jingyu＞Hunchun＞Fusong＞Ji’an＞Changbai.［Conclusions］ Endophytes in American ginseng were characterized by abundant genetic diversity. The structure of the endophytes community in American ginseng was complicated，there were differences of the endophytes community structure of different producing area and colonization part，the community diversity of producing area with high altitude was decreased.

Key words:Keywords: American ginseng，endophytes，isolation，identification，diversity，community structure