Genetic diversity analysis of Fusarium oxysporum f.sp.cubense populations from China using Inter-Simple Sequence Repeats-PCR (ISSR-PCR) technique

Abstract:

Abstract:［Objective］We used Inter-Simple Sequence Repeats (ISSR) markers to reveal the genetic diversity of 95 Fusarium oxysporum f．sp．cubense (FOC) isolates from banana in China，for the rational control of the disease．［Methods］ Eight primers were chosen for analyzing FOC isolates to study their genetic diversity by ISSR-PCR． All isolates were clustered using Unweighted Pair-Group Method with Arithmetic means ( UPGMA) analysis by NTSYSpc v2.10e software.［Results］A total of 52 sites were generated，among them 92. 3% were polymorphic．Genetic distance was 0.57 to 1.00 based on the Nei’s standard． Isolates were grouped into six distinct clusters (A，B，C，D，E and F)based on ISSR analysis using a genetic distance threshold of 0.68，the proportion of 51.06%，39.58%，5.20%，2.08%，1.04%，and 1.04%，respectively.［Conclusion］There were high levels of genetic variation among the FOC isolates，and the ISSR clustering groups had obvious correlation with hosts and races of the pathogen.

Key words:Keywords: Fusarium oxysporum f．sp．cubene，genetic diversity，ISSR