Cloning，expression，purification and characterization of two uracil-DNA glycosylases from Sulfolobus acidocaldarius

doi:10.13343/j.cnki.wsxb.20140468

Home > Archive>Volume 55, Issue 8, 2015 >1036-1041. DOI:10.13343/j.cnki.wsxb.20140468

Cloning，expression，purification and characterization of two uracil-DNA glycosylases from Sulfolobus acidocaldarius
DOI:
                        10.13343/j.cnki.wsxb.20140468
                    
CSTR:
                        32112.14.j.AMS.20140468
                    
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Fund Project:Supported by the National Natural Science Foundation of China (J1210047) and by the Shanghai Municipal Natural Science Foundation (12ZR1413700)

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Abstract: ［Objective］ To characterize uracil-DNA glycosylase from acidophilic and thermophilic Sulfolobus acidocaldarius.［Methods］We cloned udgIV and udgV genes from S.acidocaldarius，expressed the two recombinant UDG proteins in E.coli species BL21 (DE3) Rosetta-pLysS，purified the recombinant UDGs and characterized the removal of dU by UDGs.［Results］We successfully expressed two S.acidocaldarius UDGs and found both UDGs having the activity of dU removal. In comparison to UDGV，UDGIV was more efficient in dU removal，with a 750-foldactivity.［Conclusion］In comparison to UDGV，UDGIV from S． acidocaldarius was a more efficient enzyme responsible for the removal of dU from DNA in vitro.

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Jing Wang, Gangshun Yi, Jie Ou, Jianhua Liu, Xipeng Liu. Cloning，expression，purification and characterization of two uracil-DNA glycosylases from Sulfolobus acidocaldarius. [J]. Acta Microbiologica Sinica, 2015, 55(8): 1036-1041

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Received:October 02,2014
Revised:March 11,2015
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Online: July 27,2015
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