Regulation of rsc1285 gene in type III secretion system in Ralstonia solanacearum

Abstract:

Abstract:［Objective］ Rsc1285 is one of the putative T3SS-regulated factors in Ralstonia solanacearum，and the regulation of Rsc1285 on T3SS and pathogenicity was characterized.［Methods］ The rsc1285 deletion mutants were constructed by homologous recombination and characterized by complementation.［Results］ The rsc1285 mutant was significantly less virulent than the wild-type strain to infect tomato plants. Rsc1285 controls the expression of hrpB and HrpB-regulating genes，but it is dispensable for the expression of hrpG and prhG.［Conclusion］R.solanacearum uses Rsc1285 to control the T3SS and pathogenicity via a novel pathway，and this finding provides insights into overall infection mode of R.solanacearum.