Community diversity of ammonia-oxidizing bacteria of three plants rhizosphere in Ebinur Lake wetland

Abstract:

Abstract:［Objective］ In order to study the community diversity of rhizosphere soil ammonia-oxidizing bacteria，Halocnemum strobilaceum，Ｒeed and Salicornia in Ebinur Lake Wetland were investigated．［Methods］The clone libraries of amoA gene were constructed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP)，and phylogenetics were analyzed．To explore the community structure of rhizosphere amomonia-oxidizing bacteria，we combined rhizosphere physicochemical factors of the three plants．［Results］ Phylogenetic analysis of the amoA gene fragments showed that all of AOB sequences from shrimp of three plants rhizosphere were affiliated with Nitrosomonas or Nitrosomonas-like phyla，Nitrosospira phyla was not discovered． Three plants rhizosphere composition includes 9 OTUs，12 OTUs and 7 OTUs respectively． Coverages of all libraries of the three plants rhizosphere were over 99% and strongly representative． The richness index，chao1 index，ACE index and Shannon index of the three libraries were as follows，Reed rhizosphere AOB was much higher than Halocnemum strobilaceum rhizosphere AOB，and Salicornia rhizosphere AOB was the lowest．［Conclusion］ This study provides a basis for understanding the community diversity and structure of rhizosphere soil ammonia-oxidizing bacteria in Ebinur Lake wetland.

Key words:Keywords: rhizosphere，ammonia-oxidizing bacteria(AOB)，amoA gene，PCR-RFLP analysis，clone library