Yichao Zhao
College of Veterinary Medicine，Yangzhou University，Yangzhou 225009，Jiangsu Province，ChinaShaohui Wang
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaDenghui Yang
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaXin Liu
College of Veterinary Medicine，Yangzhou University，Yangzhou 225009，Jiangsu Province，ChinaXiangan Han
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaMingxing Tian
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaChan Ding
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaZongping Liu
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaShengqing Yu
Shanghai Veterinary Research Institute，Chinese Academy of Agricultural Sciences (CAAS)，Shanghai 200241，ChinaSupported by the Funds of Chinese Special Fund for Agro-scientific Research in the Public Interest (201303044)，by the National Natural Science Foundation of China (81201266，31370045) and by the National Basic Fund for Institutes，which is supported by Shanghai Veterinary Research Institute (2015JB08)，and by the Shandhai Key Project on Agricultural Development through Science and Technology (2015HNG1－9)
Abstract:［Objective］ To determined the role of vacuolating autotransporter toxin (vat) gene in avian pathogenic Escherichia coli (APEC)，we detected the biological characteristics and pathogenicity of vat gene mutant strain of APECO1．［Methods］We constructed the vat mutant and complementary strain of APEC-O1 by the Red recombination system and plasmid pSTV28． Then we compared the growth curve，motility，agglutination，biofilm formation and pathogenicity of mutant strain，wild-type strain and complementary strain．［Results］ The vat mutant did not affect the growth and resistance to environment stress of APEC．However，inactivation of APEC-O1 vat gene resulted in enhanced motility， diminished agglutination，decreased biofilm formation and attenuated virulence in ducks．［Conclusion］ These data indicated that Vat affect the motility，agglutination，biofilm formation and virulence of APEC-O1，which help us to understand the role of the Vat in the APEC pathogenicity．
Yichao Zhao, Shaohui Wang, Denghui Yang, Xin Liu, Xiangan Han, Mingxing Tian, Chan Ding, Zongping Liu, Shengqing Yu. Vacuolating autotransporter toxin affects biological characteristics and pathogenicity of avian pathogenic Escherichia coli. [J]. Acta Microbiologica Sinica, 2015, 55(9): 1208-1214
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