Screening and identification of a bacterium capable of converting agar to neoagaro oligosaccharides

Abstract:

Abstract:［Objective］ To screen and identify a bacterium capable of converting agar to neoagaro oligosaccharides．［Methods］We took samples of porphyra haitanensis and nearby seawater，and then used the medium containing 1‰ agar to enrich the target bacteria． The target isolates were obtained by dilution-plate method，of which crude enzymes were further obtained by liquid culture． We adopted DNS method to determine the target bacteria which can convert agar to neoagaro oligosaccharides． The phylogenetics was identified by analyzing 16S rDNA sequence and combining the strain’smorphological and bacterial colonial physiological biochemical characteristics．［Results］ We isolated a gram-negative bacterial strain HJPHYXJ-1 capable of transforming agar to neoagaro oligosaccharides． Basic Local Alignment Search Tool (BLAST) search of HJPHYXJ-1's 16S rDNA sequence on GenBank suggested that the similarity between this strain and Vibrio natriegens reached 99%．In addition， the morphological and physiological biochemical characteristics of HJPHYXJ-1 also showed highly similarity to Vibrio natriegens．So we identified HJPHYXJ-1 as Vibrio natriegens．The results of HPLC suggested that the metabolite of enzymatic degradation was neoagaro oligosaccharides． ［Conclusions］HJPHYXJ-1 or the new isolate of Vibrio natriegens was capable of converting agar to neoagaro oligosaccharides．

Key words:Keywords: agar，neoagaro oligosaccharides，enzymatic degradation，Vibrio natriegens