Hao He
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaYingling Zhu
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaLiqing Chi
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaZizhao Zhao
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaTing Wang
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaMingxing Zuo
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaTong Zhang
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaFengjuan Zhou
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaLiqiu Xia
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaXuezhi Ding
State Key Laboratory Breeding Base of Microbial Molecular Biology，College of Life Science，Hunan Normal University，Changsha 410081，Hunan Province，ChinaSupported by the National Natural Science Foundation of China (31370116)，by the National High Technology Research and Development program (863) of China (2011AA10A203)，by the Key Project of Hunan Provincial Education Department (13CY002，10CY013)，by he Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province (20134486) and by Training Plan to Innovation and Enterprise of National University Students (201410542009)
Abstract:［Objective］We isolated 339 bacillus strains from 72 soil samples all over the country，then purified their antimicrobial compounds and studied the antibacterial activity，to enrich bacillus resources and explore their second metabolites.［Methods］A bacillus strain with strong antibacterial activity was selected by dilution plate and water bath heating from a soil sample from a peanut plantation in Henan Province; this strain was identified according to morphological observation，physiological and biochemical characteristics，and consequences of 16S rRNA homologous analysis． Antibacterial compound from the identified strain，Bacillus amyloliquefaciens X030，was separated and purified by acetone precipitation，Sephadex chromatography，C18 reverse phase column chromatography．Its molecular weight was analyzed by LC-MS/MS． The antibacterial activity was characterized by disc diffusion and plate two-way cultivation．［Results］Bacillus amyloliquefaciens was isolated that not only has antibacterial activity against Staphylococcus aureus，Candida albican and Saccharomycetes; but also against Pyriculariaoryzae，Chili pointed cell anthrax，Gloeosporium eriobotryae speg and Phytophthora parasitica．The compound was confirmed as polypeptide．［Conclusion］ Bacillus amyloliquefaciens X030 can produce a polypeptide that inhibits pathogenic bacteria and plant pathogenic fungi．
Hao He, Yingling Zhu, Liqing Chi, Zizhao Zhao, Ting Wang, Mingxing Zuo, Tong Zhang, Fengjuan Zhou, Liqiu Xia, Xuezhi Ding. Screening and antibacterial function of Bacillus amyloliquefaciens X030. [J]. Acta Microbiologica Sinica, 2015, 55(9): 1133-1143
Copy
WeChat ID 
Mobile Terminal Acta Microbiologica Sinica ® 2025 All Rights Reserved
