Composition diversity and metabolic characters of lactic acid bacteria community SGL

Abstract:

Abstract:［Objective］We aimed to select a stable lactic acid bacteria community from switchgrass silage，that was efficient in lactic acid production.［Methods］We obtained the community by continuous restricted subcultivation in MRS broth，and analysed the composition diversity and stability of the community by 16S rRNA gene-based pyrosequencing and Denaturing Gradient Gel Electrophoresis (DGGE)，respectively．In addition，we studied the effect of different nitrogen sources on growth and lactic acid production of the community，through adding different concentrations of yeast extraction，different nitrogen sources［yeast extract，peptone，urea and (NH4)2SO4］and different proportions of (NH4 )2SO4 and yeast extract leveled with elemental nitrogen 1.8 g/L.［Results］The microbial composition of SGL became stable from the 8th generation according to the results of DGGE．The pH value of the MRS inoculated with SGL dropped to 3.7，and the concentration of lactic acid reached 26 g/L after 24 h cultivation．The result of the pyrosequencing showed that the major composition of SGL were Lactobacillus nantensis (78.78%)，Lactobacillus plantarum (7.92%)，Lactobacillus pantheris (5.27%)，Bacillus coagulans (4.41%) and Lactococcus lactics (3.31%)．The best supplementation of yeast extraction for SGL was 20 g/L．When the elemental nitrogen ratio of (NH4)2SO4 to yeast extract was 1:4，the growth and lactic acid production were no significant difference with 0:5 (P＜0.05).［Conclusion］SGL had a great potential of application，as an efficient inoculant for ensilage or lactic acid production． This study would offer theoretical basis for cultivate and application of SGL in production.

Key words:Keywords: switchgrass，silage，lactic acid bacteria community，pyrosequencing，nitrogen resources