New osmo-regulational promoters in the industrial yeast

Abstract:

Abstract:［Objective］The work was aimed at selecting osmo-regulated prompters possessing excellent performance for further research of the industrial yeast Candida glycerinogenes.［Methods］Promoters PCgPGI，PCgTPI，PCgZWF，PCgSTL1，PCgSTL2 and PCgSTL3 were amplified by PCR and their bioinformatics analysis of stress response elements (STREs) were conducted． We constructed integrative plasmids containing 5. 8S rDNA，a fluorescence protein gene gfp and a promoter PCgPGI，PCgTPI，PCgZWF，PCgSTL1，PCgSTL2 or PCgSTL3．The promoters’activities and osmoregulations were compared according to the results of fluorescence and qRT-PCR.［Results］PCgSTL3 had more STREs，higher transcription level，lager gfp expression and it was more sensitive to stress.［Conclusion］PCgSTL3 is an excellent induced promoter responding to hyperosmotic stress． Controlled expression of target genes can be realized using PCgSTL3 in the industrial yeast.

Key words:Keywords: Candida glycerinogenes，green fluorescent protein，key promoters of osmo-adaptation