Screening and ammoxidation characteristics of an ammonium oxidizing bacteria group

Abstract:

Abstract:［Objective］This study aimed to screen high-performance ammonia oxidizing bacteria (AOB) resistant to a high concentration of ammonia-nitrogen and low C/N ratio，for the development of novel AOB agents.［Methods］Multipoint sampling，compulsory domestication，gradient dilution of domestication liquid were conducted to screen AOB with efficient and stable ammonia-nitrogen removing ability，and effects of different factors on its ammoxidation ability including C/N ratio，shaking speed and ammonia-nitrogen concentration were studied． Dominant strains were screened and identified by morphological observation，physiological and biochemical properties test and 16S rRNA sequence analysis．［Results］Three efficient AOB were obtained，among them a micro-flora named JQ8 showed the highest activity． The ammonia-nitrogen removal rate reached 95.07% in a simulated wastewater with 17.86 mmol/L of initial ammonia-nitrogen at C/N 4 treated by JQ8 for 6 days． Moreover，its ammonia nitrogen removal rate kept above 95% and net nitrogen removing rate nearly 80% in the solution with a C/N ratio above 4 and an NH +4 -N concentration below 28. 57 mmol/L．The circuit board industry wastewater was treated using the laboratory-simulated aerobic active sludge disposal system．The removal rate of NH +4 -N and total nitrogen reached 87.8% and 67. 6% respectively after 7 days’treatment using JQ8．Defluvibacter sp． ，Paracoccus sp． and Aquamicrobium sp． were identified as the dominant strains after the composition analysis of JQ8．［Conclusion］An ammonia oxidizing bacteria consortium JQ8 screened from the landfill leachate showed a strong ammonium-nitrogen removal and endurance ability under low C /N ratio and high ammonia-nitrogen concentration，thus is probably applicable to intensify the ammonia-nitrogen removal treatment of industrial wastewater with sewage disposal system．

Key words:Keywords: ammonium oxidizing bacteria，screening，removal ammonium characteristics，strain identification