Whole-genome sequencing and analysis of inosineproducing strain Bacillus subtilis ATCC 13952

Abstract:

Abstract:［Objective］Bacillus subtilis ATCC 13952 is an inosine-producing strain．In order to study the mechanisms of inosine accumulation and offer help for molecular breeding，it is necessary to uncover the genome sequence of ATCC 13952.［Methods］Whole-genome sequencing of ATCC 13952 is carried out by Solexa and Sanger sequencing．Genome assembly，gene prediction and functional annotation，GO/COG cluster analysis and synteny analysis are done using relevant software.［Results］The complete genomic information of Bacillus subtilis ATCC 13952 is contained on a single circular chromosome of 3876276 bp with an average GC content of 45.8%．The genome sequence is deposited in the GenBank under the accession number CP009748． Comparative genomic analysis shows that ATCC 13952 should have significant genomic synteny with other Bacillus subtilis strains．On the other hand，some point mutation and deletions occurred in purine metabolism-related genes between ATCC 13952 and the standard strain.［Conclusion］The results of this study will provide a theoretical basis for subsequent further molecular breeding.

Key words:Keywords: inosine，Bacillus subtilis，whole-genome sequencing，molecular mechanism，comparative genomic analysis